Coding

Part:BBa_K5033003:Design

Designed by: Jonas Martin Westphal   Group: iGEM24_Aachen   (2024-09-16)


OncoBiotica: mFadA[B]_GSLinker_CDA[Gamma]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 115
    Illegal XhoI site found at 1396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 1341
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing this part we ensured BioBrick compatibility and codon optimization for Expression in E. coli.


Source

mFadA B-domain from part BBa_K4990002.

Synthetic flexible linker.

Mutant of the codA cytosine deaminase which is native to E. coli. The mutant has been ordered at a synthesysing company.

Reference

CodA at UniProt: https://www.uniprot.org/uniprotkb/P25524/entry